Journal: International Journal of Environmental Research and Public Health

Article Title: Spatial Variability of PAHs and Microbial Community Structure in Surrounding Surficial Soil of Coal-Fired Power Plants in Xuzhou, China

doi: 10.3390/ijerph13090878

Figure Lengend Snippet: PAHs concentrations and TOM content in each soil sample (see ).

Article Snippet: A mixture of 16 USEPA priority PAHs standards solution [naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorene (Fle), phenanthrene (Phe), anthracene(Anth), fluoranthene (Fla), pyrene (Pyr), benzo[a]anthracene (BaA), chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), indeno[1,2,3-c,d]pyrene (Ind), dibenz [a,h]anthracene (DBA), and benzo[g,h,i]perylene (BgP)] was purchased from TCI Development Co., Ltd. (Beijing, China).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Spatial Variability of PAHs and Microbial Community Structure in Surrounding Surficial Soil of Coal-Fired Power Plants in Xuzhou, China

doi: 10.3390/ijerph13090878

Figure Lengend Snippet: Triangle graph of percentage density for 15 PAHs in the sampling sites.

Article Snippet: A mixture of 16 USEPA priority PAHs standards solution [naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorene (Fle), phenanthrene (Phe), anthracene(Anth), fluoranthene (Fla), pyrene (Pyr), benzo[a]anthracene (BaA), chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), indeno[1,2,3-c,d]pyrene (Ind), dibenz [a,h]anthracene (DBA), and benzo[g,h,i]perylene (BgP)] was purchased from TCI Development Co., Ltd. (Beijing, China).

Techniques: Sampling

Journal: International Journal of Environmental Research and Public Health

Article Title: Spatial Variability of PAHs and Microbial Community Structure in Surrounding Surficial Soil of Coal-Fired Power Plants in Xuzhou, China

doi: 10.3390/ijerph13090878

Figure Lengend Snippet: Source analysis of PAHs through diagnostic ratios. ( a ) Plot of Fla/Pry vs. Phe/Anth; ( b ) Plot of Fla/(Fla + Pyr) vs. Ind/(Ind + BgP).

Article Snippet: A mixture of 16 USEPA priority PAHs standards solution [naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorene (Fle), phenanthrene (Phe), anthracene(Anth), fluoranthene (Fla), pyrene (Pyr), benzo[a]anthracene (BaA), chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), indeno[1,2,3-c,d]pyrene (Ind), dibenz [a,h]anthracene (DBA), and benzo[g,h,i]perylene (BgP)] was purchased from TCI Development Co., Ltd. (Beijing, China).

Techniques: Diagnostic Assay

Journal: The Journal of Immunology Author Choice

Article Title: Aryl Hydrocarbon Receptor Activation in Pulmonary Alveolar Epithelial Cells Limits Inflammation and Preserves Lung Epithelial Cell Integrity

doi: 10.4049/jimmunol.2300325

Figure Lengend Snippet: Functional AHR expression in murine AECs. (A) Top panel: 12 µg human (lanes 1 and 2) and mouse (lanes 3 and 4) whole-cell extracts separated on SDS-PAGE gel and blotted with RRID:AB_2938321 for AHR protein expression. (B) Top panel: time course of Cyp1a1 expression in MLE-15 cells following FICZ (blue lines) and PAH (red lines) stimulation. Bottom panel: time course of Ahrr expression following FICZ and PAH stimulation; n = 3 with mean values plotted. (C) Cyp1a1 and (D) Nqo1 mRNA transcript levels following control, PAH (1 µg/ml), FICZ (100 nM), BaP (10 µM), or pyrene (10 µM) exposure for 24 h. Data are representative of three independent experiments; statistical evaluation was conducted with ANOVA followed by Tukey post hoc test.

Article Snippet: AHR ligands A standardized mixture of polycyclic aromatic hydrocarbons (PAH; 2 mg/ml in methylene chloride) was supplied by Restek (Bellefonte, PA; catalog no. 31011, calibration mix 5/610 PAH).

Techniques: Functional Assay, Expressing, SDS Page, Control

Journal: The Journal of Immunology Author Choice

Article Title: Aryl Hydrocarbon Receptor Activation in Pulmonary Alveolar Epithelial Cells Limits Inflammation and Preserves Lung Epithelial Cell Integrity

doi: 10.4049/jimmunol.2300325

Figure Lengend Snippet: AHR KD modulates epithelial cell integrity. (A) Western blot confirmation of lentiviral shRNA KD of AHR protein in primary murine AECs using a 1:200 dilution of RRID:AB_2938321 Ab. The t test indicates p = 0.03. (B) RT-PCR measurement of mesenchymal (left panel) and epithelial cell markers (right panel) of control AECs versus shRNA KD of AHR in AECs. Data shown are representative of two independent experiments with replicates as shown. Statistical evaluation was conducted using a t test, with p values indicated. (C) Western blot confirmation of CRISPR-Cas9 KD of AHR activity in MLE-15 cells; densitometric quantification of clones is shown in panels (i)–(iii). Pairwise comparisons were conducted with a t test; p < 0.05 as indicated. (D) ELISA validation of AHR protein levels in whole-cell extracts from wild-type MLE-15 cells, Crispr-Cas9 cloned nontargeting (2082) control cells, and three CRISPR-Cas9 AHR target-specific clones (2669-33-1, 2669-33-2, and 2670-49-1); two extractions per cell type. (E) CYP1A1 activity was measured following 24-h exposure to PAH (1 µg/ml), FICZ (100 nM), BaP (10 µM), or pyrene (10 µM). RLU, relative light units; n = 3. The expression of (F–H) aSma, Col4a1, and Sdc1 mRNA is shown in control and CRISPR-Cas9 AHR KD MLE-15 clones; n = 3; multiple comparison analysis was conducted using ANOVA followed by Tukey post hoc test, with p values indicated.

Article Snippet: AHR ligands A standardized mixture of polycyclic aromatic hydrocarbons (PAH; 2 mg/ml in methylene chloride) was supplied by Restek (Bellefonte, PA; catalog no. 31011, calibration mix 5/610 PAH).

Techniques: Western Blot, shRNA, Reverse Transcription Polymerase Chain Reaction, Control, CRISPR, Activity Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Expressing, Comparison

Journal: The Journal of Immunology Author Choice

Article Title: Aryl Hydrocarbon Receptor Activation in Pulmonary Alveolar Epithelial Cells Limits Inflammation and Preserves Lung Epithelial Cell Integrity

doi: 10.4049/jimmunol.2300325

Figure Lengend Snippet: AHR modulates epithelial cell wound closure. (A) The effect of AHR KD on MLE-15 cell wound closure over 48 h in a scratch model. The slope of the percentage wound confluence (representative of three individual experiments) is shown. (B) Representative images of the control and AHR monolayers at day 1 and day 2. Scale bar, 400 µm; dashed outlines indicate the areas used for quantification. A time-lapse video of wound recovery can be viewed in a supplemental file: https://doi.org/10.6084/m9.figshare.25343233. (C) The effect of AHR ligands PAH (1 µg/ml), BaP (10 µM), and FICZ (100 ng/ml) on wound closure in MLE-15 cells with intact AHR signaling (CRISPR control) cell wound closure. Data are representative of three independent experiments.

Article Snippet: AHR ligands A standardized mixture of polycyclic aromatic hydrocarbons (PAH; 2 mg/ml in methylene chloride) was supplied by Restek (Bellefonte, PA; catalog no. 31011, calibration mix 5/610 PAH).

Techniques: Control, CRISPR